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imp β  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology imp β
    Translocation of Cx43 to the nucleus depends on Importin-β. ( a ) Cx43 was IP from purified nuclear or whole cell extracts, after which interaction between Cx43 and Importin-β was analysed by WB. ( b ) HEK293 Cx43+ cells were transfected with GFP-Importin-β <t>(GFP-IMP</t> β ) for 24 h, after which co-localization between Cx43 and GFP-Importin-β was analysed by confocal microscopy. Line profile plots depict the intensity distribution of green and magenta channels along the dashed white lines in the merged image. Arrows in co-localization plots indicate the margins of the nucleus. Inset images display a magnified view of the boxed region. Scale bars, 10 µm. ( c ) siRNA-mediated knockdown of Importin-β was performed in HEK293 Cx43+ cells, for 48 h. Sucrose cushion-based nuclei purification was performed, followed by WB analysis of Cx43. Graphs depict quantification of nuclear and input Cx43 levels, normalized by total protein levels (Ponceau staining; n = 4 biological replicates). ( d ) Levels of nuclei-localized Cx43 WT or Cx43 ΔCT were assessed by WB in purified nuclei. Graphs depict quantification of nuclear Cx43, normalized for transfected Cx43 (input levels; n = 4 biological replicates). ( e ) V5 was IP from whole-cell extracts of HEK293 Cx43− cells transfected with V5-tagged full-length (Cx43 WT ) or NLS-deleted Cx43 (Cx43 ΔNLS ) for 24 h. Graph shows interaction levels of importin-β with Cx43 (V5) assessed by WB ( n = 5 biological replicates). n.s: non-significant.
    Imp β, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imp β/product/Santa Cruz Biotechnology
    Average 92 stars, based on 17 article reviews
    imp β - by Bioz Stars, 2026-03
    92/100 stars

    Images

    1) Product Images from "Cx43 can form functional channels at the nuclear envelope and modulate gene expression in cardiac cells"

    Article Title: Cx43 can form functional channels at the nuclear envelope and modulate gene expression in cardiac cells

    Journal: Open Biology

    doi: 10.1098/rsob.230258

    Translocation of Cx43 to the nucleus depends on Importin-β. ( a ) Cx43 was IP from purified nuclear or whole cell extracts, after which interaction between Cx43 and Importin-β was analysed by WB. ( b ) HEK293 Cx43+ cells were transfected with GFP-Importin-β (GFP-IMP β ) for 24 h, after which co-localization between Cx43 and GFP-Importin-β was analysed by confocal microscopy. Line profile plots depict the intensity distribution of green and magenta channels along the dashed white lines in the merged image. Arrows in co-localization plots indicate the margins of the nucleus. Inset images display a magnified view of the boxed region. Scale bars, 10 µm. ( c ) siRNA-mediated knockdown of Importin-β was performed in HEK293 Cx43+ cells, for 48 h. Sucrose cushion-based nuclei purification was performed, followed by WB analysis of Cx43. Graphs depict quantification of nuclear and input Cx43 levels, normalized by total protein levels (Ponceau staining; n = 4 biological replicates). ( d ) Levels of nuclei-localized Cx43 WT or Cx43 ΔCT were assessed by WB in purified nuclei. Graphs depict quantification of nuclear Cx43, normalized for transfected Cx43 (input levels; n = 4 biological replicates). ( e ) V5 was IP from whole-cell extracts of HEK293 Cx43− cells transfected with V5-tagged full-length (Cx43 WT ) or NLS-deleted Cx43 (Cx43 ΔNLS ) for 24 h. Graph shows interaction levels of importin-β with Cx43 (V5) assessed by WB ( n = 5 biological replicates). n.s: non-significant.
    Figure Legend Snippet: Translocation of Cx43 to the nucleus depends on Importin-β. ( a ) Cx43 was IP from purified nuclear or whole cell extracts, after which interaction between Cx43 and Importin-β was analysed by WB. ( b ) HEK293 Cx43+ cells were transfected with GFP-Importin-β (GFP-IMP β ) for 24 h, after which co-localization between Cx43 and GFP-Importin-β was analysed by confocal microscopy. Line profile plots depict the intensity distribution of green and magenta channels along the dashed white lines in the merged image. Arrows in co-localization plots indicate the margins of the nucleus. Inset images display a magnified view of the boxed region. Scale bars, 10 µm. ( c ) siRNA-mediated knockdown of Importin-β was performed in HEK293 Cx43+ cells, for 48 h. Sucrose cushion-based nuclei purification was performed, followed by WB analysis of Cx43. Graphs depict quantification of nuclear and input Cx43 levels, normalized by total protein levels (Ponceau staining; n = 4 biological replicates). ( d ) Levels of nuclei-localized Cx43 WT or Cx43 ΔCT were assessed by WB in purified nuclei. Graphs depict quantification of nuclear Cx43, normalized for transfected Cx43 (input levels; n = 4 biological replicates). ( e ) V5 was IP from whole-cell extracts of HEK293 Cx43− cells transfected with V5-tagged full-length (Cx43 WT ) or NLS-deleted Cx43 (Cx43 ΔNLS ) for 24 h. Graph shows interaction levels of importin-β with Cx43 (V5) assessed by WB ( n = 5 biological replicates). n.s: non-significant.

    Techniques Used: Translocation Assay, Purification, Transfection, Confocal Microscopy, Knockdown, Staining



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    imp β  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology imp β
    Translocation of Cx43 to the nucleus depends on Importin-β. ( a ) Cx43 was IP from purified nuclear or whole cell extracts, after which interaction between Cx43 and Importin-β was analysed by WB. ( b ) HEK293 Cx43+ cells were transfected with GFP-Importin-β <t>(GFP-IMP</t> β ) for 24 h, after which co-localization between Cx43 and GFP-Importin-β was analysed by confocal microscopy. Line profile plots depict the intensity distribution of green and magenta channels along the dashed white lines in the merged image. Arrows in co-localization plots indicate the margins of the nucleus. Inset images display a magnified view of the boxed region. Scale bars, 10 µm. ( c ) siRNA-mediated knockdown of Importin-β was performed in HEK293 Cx43+ cells, for 48 h. Sucrose cushion-based nuclei purification was performed, followed by WB analysis of Cx43. Graphs depict quantification of nuclear and input Cx43 levels, normalized by total protein levels (Ponceau staining; n = 4 biological replicates). ( d ) Levels of nuclei-localized Cx43 WT or Cx43 ΔCT were assessed by WB in purified nuclei. Graphs depict quantification of nuclear Cx43, normalized for transfected Cx43 (input levels; n = 4 biological replicates). ( e ) V5 was IP from whole-cell extracts of HEK293 Cx43− cells transfected with V5-tagged full-length (Cx43 WT ) or NLS-deleted Cx43 (Cx43 ΔNLS ) for 24 h. Graph shows interaction levels of importin-β with Cx43 (V5) assessed by WB ( n = 5 biological replicates). n.s: non-significant.
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    Image Search Results


    Translocation of Cx43 to the nucleus depends on Importin-β. ( a ) Cx43 was IP from purified nuclear or whole cell extracts, after which interaction between Cx43 and Importin-β was analysed by WB. ( b ) HEK293 Cx43+ cells were transfected with GFP-Importin-β (GFP-IMP β ) for 24 h, after which co-localization between Cx43 and GFP-Importin-β was analysed by confocal microscopy. Line profile plots depict the intensity distribution of green and magenta channels along the dashed white lines in the merged image. Arrows in co-localization plots indicate the margins of the nucleus. Inset images display a magnified view of the boxed region. Scale bars, 10 µm. ( c ) siRNA-mediated knockdown of Importin-β was performed in HEK293 Cx43+ cells, for 48 h. Sucrose cushion-based nuclei purification was performed, followed by WB analysis of Cx43. Graphs depict quantification of nuclear and input Cx43 levels, normalized by total protein levels (Ponceau staining; n = 4 biological replicates). ( d ) Levels of nuclei-localized Cx43 WT or Cx43 ΔCT were assessed by WB in purified nuclei. Graphs depict quantification of nuclear Cx43, normalized for transfected Cx43 (input levels; n = 4 biological replicates). ( e ) V5 was IP from whole-cell extracts of HEK293 Cx43− cells transfected with V5-tagged full-length (Cx43 WT ) or NLS-deleted Cx43 (Cx43 ΔNLS ) for 24 h. Graph shows interaction levels of importin-β with Cx43 (V5) assessed by WB ( n = 5 biological replicates). n.s: non-significant.

    Journal: Open Biology

    Article Title: Cx43 can form functional channels at the nuclear envelope and modulate gene expression in cardiac cells

    doi: 10.1098/rsob.230258

    Figure Lengend Snippet: Translocation of Cx43 to the nucleus depends on Importin-β. ( a ) Cx43 was IP from purified nuclear or whole cell extracts, after which interaction between Cx43 and Importin-β was analysed by WB. ( b ) HEK293 Cx43+ cells were transfected with GFP-Importin-β (GFP-IMP β ) for 24 h, after which co-localization between Cx43 and GFP-Importin-β was analysed by confocal microscopy. Line profile plots depict the intensity distribution of green and magenta channels along the dashed white lines in the merged image. Arrows in co-localization plots indicate the margins of the nucleus. Inset images display a magnified view of the boxed region. Scale bars, 10 µm. ( c ) siRNA-mediated knockdown of Importin-β was performed in HEK293 Cx43+ cells, for 48 h. Sucrose cushion-based nuclei purification was performed, followed by WB analysis of Cx43. Graphs depict quantification of nuclear and input Cx43 levels, normalized by total protein levels (Ponceau staining; n = 4 biological replicates). ( d ) Levels of nuclei-localized Cx43 WT or Cx43 ΔCT were assessed by WB in purified nuclei. Graphs depict quantification of nuclear Cx43, normalized for transfected Cx43 (input levels; n = 4 biological replicates). ( e ) V5 was IP from whole-cell extracts of HEK293 Cx43− cells transfected with V5-tagged full-length (Cx43 WT ) or NLS-deleted Cx43 (Cx43 ΔNLS ) for 24 h. Graph shows interaction levels of importin-β with Cx43 (V5) assessed by WB ( n = 5 biological replicates). n.s: non-significant.

    Article Snippet: Non-targeting sequences (Ambion, Thermo Fisher Scientific) were used as controls. siRNA against human YBX1 (siGENOME (4904) siRNA SMARTpool) were obtained from Horizon Discovery (Dharmacon, Lafayette, CO, USA). siRNA against ERp29 (sc-60599) and IMP β (sc-35736) were obtained from SCBT.

    Techniques: Translocation Assay, Purification, Transfection, Confocal Microscopy, Knockdown, Staining